Abstract
Cryo-transmission electron microscopy (Cryo-TEM), and particularly Single Particle Analysis,
(SPA), is rapidly becoming the premier method for determining the three-dimensional structure of protein complexes and viruses. In the last several years there have been dramatic technological improvements in Cryo-TEM, such as advancements in automation and use of improved direct electron detectors, as well as improved image processing techniques.

Furthermore, it is now becoming possible to determine the structure of proteins in situ with Cryo-Electron Tomography using of focused ion beam milled thin samples. This aspect of Cryo-TEM opens 200 – 300 nm thin windows into the cell’s interior, enabling targeting of structural features within the cellular context. Please join this informative session to learn how Cryo-TEM workflows capable of generating nearly atomic resolution structures can be used to solve molecular and structural biology challenges.