The 4D-Nucleofector System is suited to handle efficient transfection or electroporation of hard-to-transfect cell lines and primary cells in 100 µL cuvettes or 20 µL 16-well NucleocuvetteTM Stripsstrips. The medium-throughput 96-well shuttle add-on for the 4D-NucleofectorTM System is also suited for transfection of hard-to-transfect cell lines or primary cells in 96-well plates.

he 4D-NucleofectorTM Core Unit is the controlling unit of the 4D-NucleofectorTM System. The different functional units can be added in a modular way to the 4D-NucleofectorTM Core Unit and each is suited for different applications:
 

• X Unit: Supporting Nucleofection of various cell numbers in different formats (16-well NucleocuvetteTM Strips or 100 µL NucleocuvetteTM Vessels 
• Y Unit: Enabling adherent Nucleofection in 24-well culture plates 
• LV Unit: Large-scale transfection of up to 1x109 cells 
• 96-well ShuttleTM Add-on: Higher throughput Nucleofection in 96-well NucleocuvetteTM Plates

Transfections per minute:

• X Unit (16-well NucleocuvetteTM Strips): 16-32 reactions
• X Unit (100 µL NucleocuvetteTM Vessels): 20-26 reactions
• Y Unit (Dipping Electrodes): 8-10 reactions 
• LV Unit (LV NucleocuvetteTM Cartridges): 2-3 mL

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0
Weight: 4.4 kg ( 9.7 lb)
Power: 100 - 240 VAC
Frequency: 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environnement non condensing Voltage limitation: 1500 V
Current limitation: 100 A